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Role of Connective Tissue Growth Factor and Angiotensin II in Tubulointerstitial Fibrosis in Experimental Obstructive Nephropathy
http://hdl.handle.net/10069/9352
http://hdl.handle.net/10069/9352497b86a1-b636-4fdc-b218-32414db4865c
名前 / ファイル | ライセンス | アクション |
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acta51_01_09.pdf (2.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-11-02 | |||||
タイトル | ||||||
タイトル | Role of Connective Tissue Growth Factor and Angiotensin II in Tubulointerstitial Fibrosis in Experimental Obstructive Nephropathy | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Obstructivenephropathy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Unilateralureteralobstruction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CTGF | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | AngiotensinII | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tubulointerstitialfibrosis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Le, VietThang
× Le, VietThang× Shigematsu, Kazuto× Zha, Yan× Nazneen, Arifa× S.Razzaque, Mohammed× Taguchi, Takashi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Angiotensin II (Ang-II) and connective tissue growth factor (CTGF) are involved in various renal disorders that lead to end-stage renal disease. Here, we determined the role of Ang-II and CTGF in the progression of tubulointerstitial injury in the rat unilateral ureteral obstruction (UUO) model. Sprague-Dawley rats (n=16) were used; 10 rats underwent UUO, and 6 control rats underwent sham operation; and rats of both groups were sacrificed on days 7 or 14. Histomorphometric analysis was performed to quantitate tubulointerstitial injuries in the experimental group. Kidney sections were stained immunohistochemically for Ang-II, CTGF, transforming growth factor-β1 (TGF-β1), type III collagen and α-smooth muscle actin (α-SMA). Renal CTGF expression was studied using in situ hybridization and reverse transcriptase-polymerase chain reaction. Double staining for Ang-II with α-SMA and CTGF with α-SMA was performed to identify cells with enhanced expression of Ang-II and CTGF. Similar dual staining of Ang-II with type III collagen and CTGF with type III collagen was performed. The correlation between Ang-II and CTGF expression and tubulointerstitial injury was examined. In obstructed kidneys, there was a significant (p<0.001) increase in expression of Ang-II, CTGF, TGF-β1, type III collagen and α-SMA, compared with control kidneys. Tubular epithelial cells and interstitial cells were the main Ang-II- and CTGF-producing cells in the obstructed kidneys. A significantly (p<0.001) positive correlation was detected in obstructed kidneys between renal expression of CTGF and expression of TGF-β1 (r=0.91), type III collagen (r=0.87) or α-SMA (r=0.90). Similarly a significantly (p<0.001) positive correlation was found in obstructed kidneys between Ang-II expression and expression of TGF-β1 (r=0.88), type III collagen (r=0.79) and α-SMA (r=0.91). Finally, there were significantly positive correlations between CTGF /Ang-II expression and tubulointerstitial fibrosis in the obstructed kidneys (r=0.88, p<0.001). The results of our in vivo studies suggest that both Ang-II and CTGF, produced by intrarenal cells, coordinately regulate progression of renal tubulointerstitial injury, by facilitating increased accumulation of interstitial collagens in obstructed kidneys. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 51, 号 1, p. 9-17, 発行日 2006-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/51.9 | |||||
sortkey | ||||||
P00009-P00017 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 2006, 51(1), p.9-17 |