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Comparative Assay on Dengue IgM-ELISA Using Reagents From 2 Sources
http://hdl.handle.net/10069/4710
http://hdl.handle.net/10069/471086db1674-4c4e-4831-b1ab-ca59496e2052
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
tm37_03_03_t.pdf (467.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2006-04-26 | |||||
| タイトル | ||||||
| タイトル | Comparative Assay on Dengue IgM-ELISA Using Reagents From 2 Sources | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Mohamed, Halimah
× Mohamed, Halimah× Balasubramaniam, Vijayamalar× Cardosa, Jane× Sinniah, Mangalam× Igarashi, Akira× Tanaka, Hiroshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to distribute dengue IgM-capture ELISA to the peripheral laboratories, supply of key reagents should be established by local production. In this study, comparative IgM-capture ELISA was carried out on a total of 237 sera using key reagents from 2 sources. The current set consisted of suckling mouse brain (SMB)-derived dengue type 2 (D2) antigen and a D2 monoclonal antibody (MAb) supplied from the United States. While an alternative set was supplied by one of the authors, consisting of tissue culture-derived dengue antigen (TCA) and MAb established in USM. When the IgM-ELISA results were compared with those by the hemagglutination-inhibition (HI) test as a gold standard, the sensitivity was 50.0% by the current set and 75.0% by the alternative set of reagents, respectively. While the specificity was 95.2% by the current set and 90.3% by the alternative set of reagents. The results showed that the alternative set of reagents can effectively be used in dengue IgM-ELISA and is significantly more sensitive than the current set of reagents (Chi SQR=12.26, P<0.01) at less than 1% risk. However there is no significant difference in specificity between the two reagents (Chi SQR=2.45, 0.5>P>0.1) at 10% risk. | |||||
| 書誌情報 |
熱帯医学 Tropical medicine 巻 37, 号 3, p. 109-114, 発行日 1995-12-26 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03855643 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00199644 | |||||
| 出版者 | ||||||
| 出版者 | 長崎大学熱帯医学研究所 | |||||
| 出版者別言語 | ||||||
| 値 | Institute of Tropical Medicine, Nagasaki University | |||||
| sortkey | ||||||
| 値 | P00109-P00114 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 熱帯医学 Tropical medicine 37(3). p109-114, 1995 | |||||
