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Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus
http://hdl.handle.net/10069/37782
http://hdl.handle.net/10069/377824afd67a1-3c7e-413c-9264-22c184261985
名前 / ファイル | ライセンス | アクション |
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JVM249_102.pdf (941.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-09-26 | |||||
タイトル | ||||||
タイトル | Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Foot-and-Mouth disease | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | FMDV | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diagnostics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rRT-PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RT-LAMP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RT-RPA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Howson, Emma L.A.
× Howson, Emma L.A.× Kurosaki, Yohei× Yasuda, Jiro× Takahashi, Masayoshi× Goto, Hiroaki× Gray, Ashley R.× Mioulet, Valerie× King, Donald P.× Fowler, Veronica L. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study describes the first multiway comparison of portable isothermal assays for the detection of foot-and-mouth disease virus (FMDV), benchmarked against real-time reverse transcription RT-PCR (rRT-PCR). The selected isothermal chemistries included reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA). The analytical sensitivity of RT-LAMP was comparable to rRT-PCR (101 RNA copies), while RT-RPA was one log10 less sensitive (102 RNA copies). Diagnostic performance was evaluated using a panel of 35 samples from FMDV-positive cattle and eight samples from cattle infected with other vesicular viruses. Assay concordance for RT-LAMP and RT-RPA was 86?98% and 67?77%, respectively, when compared to rRT-PCR, with discordant samples consistently having high rRT-PCR cycle threshold values (no false-positives were detected for any assay). In addition, a hierarchy of sample preparation methods, from robotic extraction to simple dilution of samples, for epithelial suspensions, serum and oesophageal-pharyngeal (OP) fluid were evaluated. Results obtained for RT-LAMP confirmed that FMDV RNA can be detected in the absence of RNA extraction. However, simple sample preparation methods were less encouraging for RT-RPA, with accurate results only obtained when using RNA extraction. Although the evaluation of assay performance is specific to the conditions tested in this study, the compatibility of RT-LAMP chemistry with multiple sample types, both in the presence and absence of nucleic acid extraction, provides advantages over alternative isothermal chemistries and alternative pen-side diagnostics such as antigen-detection lateral-flow devices. These characteristics of RT-LAMP enable the assay to be performed over a large diagnostic detection window, providing a realistic means to rapidly confirm positive FMD cases close to the point of sampling. | |||||
書誌情報 |
Journal of Virological Methods 巻 249, p. 102-110, 発行日 2017-11 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01660934 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jviromet.2017.08.013 | |||||
権利 | ||||||
権利情報 | c 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Virological Methods, 249, pp.102-110; 2017 |