WEKO3
アイテム
Preferential dipeptide incorporation of Porphyromonas gingivalis mediated by proton-dependent oligopeptide transporter (Pot)
http://hdl.handle.net/10069/40457
http://hdl.handle.net/10069/40457dce11dda-82a9-4022-a332-92e5442bbe9b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FEMSML_fnaa204.pdf (3.8 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-12-24 | |||||
| タイトル | ||||||
| タイトル | Preferential dipeptide incorporation of Porphyromonas gingivalis mediated by proton-dependent oligopeptide transporter (Pot) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dipeptide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dipeptidyl-peptidase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | oligopeptide transporter | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Porphyromonas gingivalis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | proton-dependent oligopeptide transporter | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | serine/threonine transporter | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ohara-Nemoto, Yuko
× Ohara-Nemoto, Yuko× Sarwar, Mohammad Tanvir× Shimoyama, Yu× Kobayakawa, Takeshi× Nemoto, Takayuki K |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Multiple dipeptidyl-peptidases (DPPs) are present in the periplasmic space of Porphyromonas gingivalis, an asaccharolytic periodontopathic bacterium. Dipeptides produced by DPPs are presumed to be transported into the bacterial cells and metabolized to generate energy and cellular components. The present study aimed to identify a transporter responsible for dipeptide uptake in the bacterium. A real-time metabolic analysis demonstrated that P. gingivalis preferentially incorporated Gly-Xaa dipeptides, and then,single amino acids, tripeptides, and longer oligopeptides to lesser extents. Heterologous expression of the P. gingivalis serine/threonine transporter (SstT) (PGN_1460), oligopeptide transporter (Opt) (PGN_1518), and proton-dependent oligopeptide transporter (Pot) (PGN_0135) genes demonstrated that Escherichia coli expressing Pot exclusively incorporated Gly-Gly, while SstT managed Ser uptake and Opt was responsible for Gly-Gly-Gly uptake. Dipeptide uptake was significantly decreased in a P. gingivalis Δpot strain and further suppressed in a Δpot-Δopt double-deficient strain. In addition, the growth of the Δpot strain was markedly attenuated and the Δpot-Δopt strain scarcely grew, whereas the ΔsstT strain grew well almost like wild type. Consequently, these results demonstrate that predominant uptake of dipeptide in P. gingivalis is mostly managed by POT. We thus propose that Pot is a potential therapeutic target of periodontal disease and P. gingivalis related systemic diseases. |
|||||
| 書誌情報 |
FEMS Microbiology Letters p. fnaa204, 発行日 2020-12-18 |
|||||
| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03781097 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 15746968 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/femsle/fnaa204 | |||||
| 権利 | ||||||
| 権利情報 | c FEMS 2020. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in FEMS Microbiology Letters following peer review. The definitive publisher-authenticated version FEMS Microbiology Letters, fnaa204, 2020 is available online at: https://doi.org/10.1093/femsle/fnaa204. |
|||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | FEMS Microbiology Letters, article in press | |||||
