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Ebola Virus GP Activates Endothelial Cells via Host Cytoskeletal Signaling Factors
http://hdl.handle.net/10069/00041553
http://hdl.handle.net/10069/000415538bd53369-bcec-44f8-aed6-144a3b1566e6
名前 / ファイル | ライセンス | アクション |
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ISYK1435_Moni.pdf (9.5 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2022-05-26 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Ebola Virus GP Activates Endothelial Cells via Host Cytoskeletal Signaling Factors | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ebola GP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ICAM-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | virus–host interactions | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytoskeletal signaling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | focal adhesion kinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TNF-α | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | host factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antivirals | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | micropinocytosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rho signaling pathway | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Moni , Mpia Benedicte
× Moni , Mpia Benedicte |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Ebola virus disease (EVD) is a lethal disease caused by the highly pathogenic Ebola virus (EBOV), and its major symptoms in severe cases include vascular leakage and hemorrhage. These symptoms are caused by abnormal activation and disruption of endothelial cells (ECs) whose mediators include EBOV glycoprotein (GP) without the need for viral replication. However, the detailed molecular mechanisms underlying virus–host interactions remain largely unknown. Here, we show that EBOV-like particles (VLPs) formed by GP, VP40, and NP activate ECs in a GP-dependent manner, as demonstrated by the upregulation of intercellular adhesion molecules-1 (ICAM-1) expression. VLPs-mediated ECs activation showed a different kinetic pattern from that of TNF-α-mediated activation and was associated with apoptotic ECs disruption. In contrast to TNF-α, VLPs induced ICAM-1 overexpression at late time points. Furthermore, screening of host cytoskeletal signaling inhibitors revealed that focal adhesion kinase inhibitors were found to be potent inhibitors of ICAM-1 expression mediated by both TNF-α and VLPs. Our results suggest that EBOV GP stimulates ECs to induce endothelial activation and dysfunction with the involvement of host cytoskeletal signaling factors, which represent potential therapeutic targets for EVD. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1435号 学位授与年月日:令和4年3月18日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Benedicte Mpia Moni, Yasuteru Sakurai and Jiro Yasuda | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Viruses, 14(1), art.no. 142; 2022 | |||||
書誌情報 |
Viruses 巻 14, 号 1, p. 142, 発行日 2022-03-18 |
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EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1999-4915 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/v14010142 | |||||
権利 | ||||||
権利情報 | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | エボラウイルスGPは宿主の細胞骨格シグナル伝達因子を介して内皮細胞を活性化する | |||||
出版者 | ||||||
出版者 | MDPI | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/00041438 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2022-03-18 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第1435号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2022-03-18) |