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Development of a selective fluorescence derivatization strategy for thyroid hormones based on the Sonogashira coupling reaction
http://hdl.handle.net/10069/00041660
http://hdl.handle.net/10069/000416606e7f8d11-dc57-401e-b3f7-33316431412b
名前 / ファイル | ライセンス | アクション |
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JCA1677_463275.pdf (1.5 MB)
Download is available from 2024/6/29.
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-08-05 | |||||
タイトル | ||||||
タイトル | Development of a selective fluorescence derivatization strategy for thyroid hormones based on the Sonogashira coupling reaction | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fluorescence derivatization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Thyroid hormones | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sonogashira coupling reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human serum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fluorescent alkyne | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakano-Yasaka, Naoyuki
× Nakano-Yasaka, Naoyuki× Kishikawa, Naoya× El-Maghrabey, Mahmoud× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A new fluorescence derivatization technique for the determination of the thyroid hormones, 3,3’,5-triiodo-L-thyronine (T3, triiodothyronine) and 3,3’,5,5’-tetraiodo-L-thyronine (T4, L-thyroxine), in human serum was developed based on the Sonogashira coupling reaction. This derivatization reaction was recently utilized by our research group as a promising solution for the derivatization of ortho-substituted aryl halides that suffer from steric hindrance. T3 and T4 possess amino groups that could be derivatized by many reagents; however, these reagents are not useful in the case of biological analysis as they could non-selectively react with many biogenic amines and amino acids. Thus, herein we aimed at labeling the iodo-phenyl group of T3 and T4 as a selective fluorescence labeling approach suitable for biological analysis. The fluorescent alkyne, 2-(4-ethynylphenyl)-4,5-diphenyl-1H-imidazole (DIB-ET), can label the ortho-substituted aryl halides T3 and T4 in the presence of palladium and copper as catalysts, overcoming the steric hindrance of ortho-substitution. Furthermore, the application of the proposed method for the selective analysis of T3 and T4 in biological samples was successfully performed even in the presence of numerous biological components. The formed fluorescent derivatives produced from the reaction of DIB-ET and T3 and T4 could be determined by an HPLC system with fluorescence detection. The proposed method was successfully applied for the selective and sensitive determination of T3 and T4 in human serum with the detection limits (S/N = 3) of 4.0 and 6.1 ng/mL and the recovery rate in the range of 84.3-92.1% and 81.3-84.9%, respectively. Therefore, the proposed method could be used as a new simple tool for the simultaneous determination of T3 and T4 in biological samples. | |||||
書誌情報 |
Journal of Chromatography A 巻 1677, p. 463275, 発行日 2022-06-30 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219673 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.chroma.2022.463275 | |||||
権利 | ||||||
権利情報 | © 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Chromatography A, 1677, art. no. 463275; 2022 |