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Development of an apolipoprotein E mimetic peptide–lipid conjugate for efficient brain delivery of liposomes
http://hdl.handle.net/10069/00042055
http://hdl.handle.net/10069/0004205569ac1baa-ff82-4880-a047-16e4e8e37577
名前 / ファイル | ライセンス | アクション |
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DD30_2173333.pdf (2.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2023-02-14 | |||||
タイトル | ||||||
タイトル | Development of an apolipoprotein E mimetic peptide–lipid conjugate for efficient brain delivery of liposomes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Apolipoprotein E mimetic | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | peptide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | blood–brain barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | brain-targeting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | liposomes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tissue clearing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kato, Naoya
× Kato, Naoya× Yamada, Sakura× Suzuki, Rino× Iida, Yoshiki× Matsumoto, Makoto× Fumoto, Shintaro× Arima, Hidetoshi× Mukai, Hidefumi× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Liposomes are versatile carriers that can encapsulate various drugs; however, for delivery to the brain, they must be modified with a targeting ligand or other modifications to provide blood–brain barrier (BBB) permeability, while avoiding rapid clearance by reticuloendothelial systems through polyethylene glycol (PEG) modification. BBB-penetrating peptides act as brain-targeting ligands. In this study, to achieve efficient brain delivery of liposomes, we screened the functionality of eight BBB-penetrating peptides reported previously, based on high-throughput quantitative evaluation methods with in vitro BBB permeability evaluation system using Transwell, in situ brain perfusion system, and others. For apolipoprotein E mimetic tandem dimer peptide (ApoEdp), which showed the best brain-targeting and BBB permeability in the comparative evaluation of eight peptides, its lipid conjugate with serine–glycine (SG)5 spacer (ApoEdp-SG-lipid) was newly synthesized and ApoEdp-modified PEGylated liposomes were prepared. ApoEdp-modified PEGylated liposomes were effectively associated with human brain capillary endothelial cells via the ApoEdp sequence and permeated the membrane in an in vitro BBB model. Moreover, ApoEdp-modified PEGylated liposomes accumulated in the brain 3.9-fold higher than PEGylated liposomes in mice. In addition, the ability of ApoEdp-modified PEGylated liposomes to localize beyond the BBB into the brain parenchyma in mice was demonstrated via three-dimensional imaging with tissue clearing. These results suggest that ApoEdp-SG-lipid modification is an effective approach for endowing PEGylated liposomes with the brain-targeting ability and BBB permeability. | |||||
書誌情報 |
Drug Delivery 巻 30, 号 1, p. 2173333, 発行日 2023-01-31 |
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出版者 | ||||||
出版者 | Taylor and Francis Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1071-7544 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/10717544.2023.2173333 | |||||
権利 | ||||||
権利情報 | © 2023 The Author(s). Published by informa UK limited, trading as Taylor & Francis group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Drug Delivery, 30(1), art. no. 2173333; 2023 |