WEKO3
アイテム
{"_buckets": {"deposit": "3233c98f-8cfa-4c83-a2e2-5958af8e9bc4"}, "_deposit": {"created_by": 6, "id": "3857", "owners": [6], "pid": {"revision_id": 0, "type": "depid", "value": "3857"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00003857", "sets": ["24"]}, "author_link": ["115521", "115520"], "item_5_alternative_title_19": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "フリーズドライ保存した多血小板血漿は骨再生に有用である"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-02-01", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "178", "bibliographicPageStart": "172", "bibliographicVolumeNumber": "73", "bibliographic_titles": [{"bibliographic_title": "Archives of Oral Biology"}]}]}, "item_5_date_granted_64": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2017-02-01"}]}, "item_5_degree_grantor_62": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "Nagasaki University (長崎大学)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17301", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_5_degree_name_61": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(歯学)"}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objective: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. Design: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at ?20 °C for one month. It was then rehydrated with an equal or 1/3 amount of distilled water (×1FD-PRP, ×3FD-PRP, respectively), and we assessed its gelation properties and the release of growth factors (PDGF-BB, TGF-β1, and VEGF). We also examined the bone forming ability with onlay-grafting on mice calvaria using β-TCP granules as a scaffold. Results: FD-PRP showed comparable gelation as f-PRP. In terms of growth factor release, × 1FD-PRP released identical concentrations of PDGF-BB and TGF-β1 to f-PRP, while ×3FD-PRP released approximately 3-fold concentrations when compared with f-PRP. In vivo, ×1FD-PRP promoted identical levels of the bone formation as f-PRP, and ×3FD-PRP induced more abundant bone formation. Conclusions: \nThese results suggest that f-PRP can be stored without functional loss by freeze-drying and the concentration of PRP may improve its efficacy in bone engineering.", "subitem_description_type": "Abstract"}]}, "item_5_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "長崎大学学位論文 学位記番号:博(医歯薬)甲第913号 学位授与年月日:平成29年2月1日", "subitem_description_type": "Other"}, {"subitem_description": "Author: Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina", "subitem_description_type": "Other"}, {"subitem_description": "Citation: Archives of Oral Biology, 73, pp.172-178; 2017", "subitem_description_type": "Other"}]}, "item_5_description_69": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Nagasaki University (長崎大学), 博士(歯学) (2017-02-01)", "subitem_description_type": "Other"}]}, "item_5_dissertation_number_65": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲医歯薬第913号"}]}, "item_5_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "115521", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nakatani, Yuya"}]}]}, "item_5_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_5_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.archoralbio.2016.10.006", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2016 Elsevier Ltd. All rights reserved."}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00039969", "subitem_source_identifier_type": "ISSN"}]}, "item_5_text_67": {"attribute_name": "学位の種類", "attribute_value_mlt": [{"subitem_text_value": "課程博士"}]}, "item_5_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "中谷, 佑哉"}], "nameIdentifiers": [{"nameIdentifier": "115520", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-02-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ISYK913_Nakatani.pdf", "filesize": [{"value": "2.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2300000.0, "url": {"label": "ISYK913_Nakatani.pdf", "objectType": "fulltext", "url": "https://nagasaki-u.repo.nii.ac.jp/record/3857/files/ISYK913_Nakatani.pdf"}, "version_id": "e9908738-a6ec-49c4-9e83-53dd6873a62b"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Platelet-rich plasma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Freeze-Dry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Long storage", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Super-concentrated platelets", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bone regeneration", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Efficacy of freeze-dried platelet-rich plasma in bone engineering", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Efficacy of freeze-dried platelet-rich plasma in bone engineering", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "6", "path": ["24"], "permalink_uri": "http://hdl.handle.net/10069/37042", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-02-06"}, "publish_date": "2017-02-06", "publish_status": "0", "recid": "3857", "relation": {}, "relation_version_is_last": true, "title": ["Efficacy of freeze-dried platelet-rich plasma in bone engineering"], "weko_shared_id": -1}
Efficacy of freeze-dried platelet-rich plasma in bone engineering
http://hdl.handle.net/10069/37042
http://hdl.handle.net/10069/37042eb127b20-14eb-49a6-8025-9df9976bbb3d
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-02-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Efficacy of freeze-dried platelet-rich plasma in bone engineering | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Platelet-rich plasma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Freeze-Dry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Long storage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Super-concentrated platelets | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone regeneration | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
中谷, 佑哉
× 中谷, 佑哉 |
|||||
著者別名 | ||||||
姓名 | Nakatani, Yuya | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objective: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. Design: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at ?20 °C for one month. It was then rehydrated with an equal or 1/3 amount of distilled water (×1FD-PRP, ×3FD-PRP, respectively), and we assessed its gelation properties and the release of growth factors (PDGF-BB, TGF-β1, and VEGF). We also examined the bone forming ability with onlay-grafting on mice calvaria using β-TCP granules as a scaffold. Results: FD-PRP showed comparable gelation as f-PRP. In terms of growth factor release, × 1FD-PRP released identical concentrations of PDGF-BB and TGF-β1 to f-PRP, while ×3FD-PRP released approximately 3-fold concentrations when compared with f-PRP. In vivo, ×1FD-PRP promoted identical levels of the bone formation as f-PRP, and ×3FD-PRP induced more abundant bone formation. Conclusions: These results suggest that f-PRP can be stored without functional loss by freeze-drying and the concentration of PRP may improve its efficacy in bone engineering. |
|||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第913号 学位授与年月日:平成29年2月1日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Archives of Oral Biology, 73, pp.172-178; 2017 | |||||
書誌情報 |
Archives of Oral Biology 巻 73, p. 172-178, 発行日 2017-02-01 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00039969 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.archoralbio.2016.10.006 | |||||
権利 | ||||||
権利情報 | c 2016 Elsevier Ltd. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | フリーズドライ保存した多血小板血漿は骨再生に有用である | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-02-01 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第913号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(歯学) (2017-02-01) |