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Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification
http://hdl.handle.net/10069/35289
http://hdl.handle.net/10069/35289ede54ba7-4d60-4d03-b295-69f2a77ee17e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AnaSci30_805.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-05-12 | |||||
| タイトル | ||||||
| タイトル | Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fragment analysis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Helicase-dependent amplification | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ligation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MLPA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Uno, Naoki
× Uno, Naoki× Yanagihara, Katsunori |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Multiplex ligation-dependent probe amplification (MLPA) is a widely used technique for detecting genomic structural variants. The technique is based on hybridization and ligation, followed by amplification of the ligation products. Therefore, ligation is considered a fundamental process that determines the feasibility and fidelity of MLPA. However, despite the widespread use of this technique, its reaction mechanism has not been fully analyzed. Herein, we describe a ligation-independent pathway for MLPA and introduce a ligation-independent probe amplification system that can be used to obtain amplified products without the hybridization and ligation processes. Fragment analysis revealed that the ligation-independent pathway is functional and that the capacity to discriminate single nucleotides with MLPA does not depend on ligation. These findings indicate that the feasibility and fidelity of MLPA do not rely on ligation. | |||||
| 書誌情報 |
Analytical Sciences 巻 30, 号 8, p. 805-810, 発行日 2014-08-10 |
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| 出版者 | ||||||
| 出版者 | 日本分析化学会 | |||||
| 出版者別言語 | ||||||
| 値 | Japan Society for Analytical Chemistry | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09106340 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13482246 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2116/analsci.30.805 | |||||
| 権利 | ||||||
| 権利情報 | c 2014 by The Japan Society for Analytical Chemistry | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Analytical Sciences, 30(8), pp.805-810; 2014 | |||||
