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In antibody-mediated autoimmune\ndisorders, it has been found that the autoantibodies occasionally attack membrane molecules on the cell surface, thus causing\nthe disease such as myasthenia gravis. In such cases, highly sensitive autoantibody detection technology is required for early\ndiagnosis. However, autoantibody analysis technology that is specialized for membrane molecules is still under development.\nHere we demonstrate a novel method for detecting of antibodies against the extracellular portions of multipass transmembrane\nmolecules.\nMethods: Antibodies for muscarinic acetylcholine receptor type3 (M3R) were detected with two kinds of luciferase immunoprecipitaion\nsystems (LIPS), conventional LIPS (cLIPS) and its modified application, termed modified LIPS (mLIPS). 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A modified application of the luciferase immunoprecipitation systems for detecting antibodies to the G protein-coupled receptors
http://hdl.handle.net/10069/36171
http://hdl.handle.net/10069/36171668c099a-0701-4e55-b17e-a6586cc79c96
名前 / ファイル | ライセンス | アクション |
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ActMed60_69.pdf (2.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-02-10 | |||||
タイトル | ||||||
タイトル | A modified application of the luciferase immunoprecipitation systems for detecting antibodies to the G protein-coupled receptors | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GPCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LIPS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | AChR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | luciferase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | autoantibody | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | autoimmune disease | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Mukaino, Akihiro
× Mukaino, Akihiro× Higuchi, Osamu× Nakane, Shunya× Maeda, Yasuhiro× Sakai, Waka× Nakamura, Hideki× Matsuo, Hidenori× Kawakami, Atsushi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: When multipass transmembrane molecules are located on the cell surface, there may be interaction with not only bioactive molecules but also pathogenic molecules in areas protruding outside the cell. In antibody-mediated autoimmune disorders, it has been found that the autoantibodies occasionally attack membrane molecules on the cell surface, thus causing the disease such as myasthenia gravis. In such cases, highly sensitive autoantibody detection technology is required for early diagnosis. However, autoantibody analysis technology that is specialized for membrane molecules is still under development. Here we demonstrate a novel method for detecting of antibodies against the extracellular portions of multipass transmembrane molecules. Methods: Antibodies for muscarinic acetylcholine receptor type3 (M3R) were detected with two kinds of luciferase immunoprecipitaion systems (LIPS), conventional LIPS (cLIPS) and its modified application, termed modified LIPS (mLIPS). In mLIPS, antibodies against extracellular portions of membrane molecules could be preferentially detected. Results: An antibody to the amino-terminal portion of human M3R was detected with modified LIPS with a high sensitivity. In contrast, an antibody to the carboxyl-terminal portion was not detected with mLIPS, because it did not interact with intracellular portions of M3R in living cells. We also found antibodies for M3R in a patient serum with Sjogren’s syndrome. Conclusion: Our technology has a promising future, and we hope that it will be applied in the analysis of antibodies against a diverse range of multipass transmembrane molecules, including GPCRs. |
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書誌情報 |
Acta medica Nagasakiensia 巻 60, 号 2, p. 69-76, 発行日 2016-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Nagasaki University School of Medicine | |||||
出版者別言語 | ||||||
長崎大学医学部 | ||||||
sortkey | ||||||
6 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia, 60(2), pp.69-76; 2016 |