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Characterization of tick-borne encephalitis virus isolated from a tick in central Hokkaido in 2017
http://hdl.handle.net/10069/40038
http://hdl.handle.net/10069/400384c6b088a-c3e9-4260-8b06-469a3477bc26
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JGV101_497.pdf (948.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-06-15 | |||||
| タイトル | ||||||
| タイトル | Characterization of tick-borne encephalitis virus isolated from a tick in central Hokkaido in 2017 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | epidemiology | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | flavivirus | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | neuropathogenicity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | tick-borne encephalitis virus | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Takahashi, Yuji
× Takahashi, Yuji× Kobayashi, Shintaro× Ishizuka, Mariko× Hirano, Minato× Muto, Memi× Nishiyama, Shoko× Kariwa, Hiroaki× Yoshii, Kentaro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Tick-borne encephalitis virus (TBEV) is a zoonotic virus in the genus Flavivirus, family Flaviviridae. TBEV is widely distributed in northern regions of the Eurasian continent, including Japan, and causes severe encephalitis in humans. Tick-borne encephalitis (TBE) was recently reported in central Hokkaido, and wild animals with anti-TBEV antibodies were detected over a wide area of Hokkaido, although TBEV was only isolated in southern Hokkaido. In this study, we conducted a survey of ticks to isolate TBEV in central Hokkaido. One strain, designated Sapporo-17-Io1, was isolated from ticks (Ixodes ovatus) collected in Sapporo city. Sequence analysis revealed that the isolated strain belonged to the Far Eastern subtype of TBEV and was classified in a different subcluster from Oshima 5-10, which had previously been isolated in southern Hokkaido. Sapporo-17-Io1 showed similar growth properties to those of Oshima 5-10 in cultured cells and mouse brains. The mortality rate of mice infected intracerebrally with each virus was similar, but the survival time of mice inoculated with Sapporo-17-Io1 was significantly longer than that of mice inoculated with Oshima 5-10. These results indicate that the neurovirulence of Sapporo-17-Io1 was lower than that of Oshima 5-10. Using an infectious cDNA clone, the replacement of genes encoding non-structural genes from Oshima 5-10 with those from Sapporo-17-Io1 attenuated the neuropathogenicity of the cloned viruses. This result indicated that the non-structural proteins determine the neurovirulence of these two strains. Our results provide important insights for evaluating epidemiological risk in TBE-endemic areas of Hokkaido. | |||||
| 書誌情報 |
Journal of General Virology 巻 101, 号 5, p. 497-509, 発行日 2020-03-05 |
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| 出版者 | ||||||
| 出版者 | Microbiology Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00221317 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 14652099 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1099/jgv.0.001400 | |||||
| 権利 | ||||||
| 権利情報 | c 2020 The Authors. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, Volume 101, Issue 5, 2020, https://doi.org/10.1099/jgv.0.001400. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of general virology, 101(5), pp.497-509; 2020 | |||||
