| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-09-09 |
| タイトル |
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|
タイトル |
HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls. |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Beta vulgaris |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Metabolic engineering |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
p-Hydroxycinnamoyl-CoA hydratase/lyase |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
p-Hydroxybenzoic acid |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Cell wall |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Rahman, Laiq ur
Kouno, Hitomi
Hashiguchi, Yuya
Yamamoto, Hirobumi
Narbad, Arjan
Parr, Adrian
Walton, Nicholas
Ikenaga, Toshihiko
Kitamura, Yoshie
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA) at about 14% on a dry weight basis. This is the best yield achieved in plant systems so far. Determination of cell-wall components liberated by alkaline hydrolysis confirmed that the ratio of pHBA to ferulic acid was considerably higher in the HCHL-expressing clones, whereas only ferulic acid was detected in a non-expressing clone. The change in cell-wall components also resulted in a decrease in tensile strength in the HCHL-expressing clones. |
| 書誌情報 |
Bioresource technology
巻 100,
号 20,
p. 4836-4842,
発行日 2009-10
|
| 出版者 |
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出版者 |
Elsevier BV |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09608524 |
| EISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1873-2976 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11522750 |
| PubMed番号 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
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|
関連識別子 |
19457657 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.biortech.2009.04.035 |
| 権利 |
|
|
権利情報 |
Copyright c 2009 Elsevier Ltd All rights reserved. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Bioresource technology, 100(20), pp.4836-4842; 2009 |
BT100_4836.pdf (750.0 kB)
