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Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection
http://hdl.handle.net/10069/20841
http://hdl.handle.net/10069/2084146a67798-799c-4b61-bf1c-7ddec77d4bf8
名前 / ファイル | ライセンス | アクション |
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Talanta78_94.pdf (648.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-01-08 | |||||
タイトル | ||||||
タイトル | Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Doxorubicin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Doxorubicinol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pharmacokinetics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Peroxyoxalate chemiluminescence detection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Photosensitization | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ahmed, Sameh
× Ahmed, Sameh× Kishikawa, Naoya× Ohyama, Kaname× Wada, Mitsuhiro× Nakashima, Kenichiro× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in the presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-μL was sufficient. Besides, it can detect accurately the low plasma concentrations. The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7?102.4% and the inter- and intra-assay RSD values in rat plasma were 2.5?8.9%. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single-dose of DXR. | |||||
書誌情報 |
Talanta 巻 78, 号 1, p. 94-100, 発行日 2009-04-15 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00399140 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11539538 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.talanta.2008.10.043 | |||||
権利 | ||||||
権利情報 | Copyright c 2008 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Talanta, 78(1), pp.94-100; 2009 |