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Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure
http://hdl.handle.net/10069/39328
http://hdl.handle.net/10069/393281ea9f845-768d-4980-854e-175d2bdea850
名前 / ファイル | ライセンス | アクション |
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VirusRes269_197643.pdf (2.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-21 | |||||
タイトル | ||||||
タイトル | Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biofilm | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HTLV-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Salivary gland epithelial cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | T cell | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakamura, Hideki
× Nakamura, Hideki× Shimizu, Toshimasa× Takatani, Ayuko× Suematsu, Takashi× Nakamura, Tatsufumi× Kawakami, Atsushi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The initial phase of the human T cell leukemia virus-1 (HTLV-1) infection of salivary gland epithelial cells (SGECs) was examined. SGECs of patients with Sjogren's syndrome (SS) and non-SS subjects were co-cultured with the HTLV-1-infected cell line HCT-5 or MOLT-4, then immunofluorescence (IF), scanning and transmission electron microscopy (SEM/TEM) were employed. The extracellular matrix and linker proteins galectin-3, agrin, and tetherin were expressed on the surfaces of both HCT-5 and MOLT-4 cells. HTLV-1 Gag-positive spots were observed on adjacent SGECs after 1 h of co-culture with HCT-5. Both in subjects with and those without SS, agrin and tetherin were co-expressed with HTLV-1 Gag on SGECs after co-culture with HCT-5,although no polarization of HTLV-1 Gag and relevant molecules was observed. SEM showed HTLV-1 virions that were found on HCT-5 were observed in the interfaces between HCT-5 cells and SGECs. TEM imaging showed that HTLV-1 virions were transmitted to SGECs at the interface with thin film-like structure, while HTLV-1 virions were released from the surface of HCT-5 cells. No endogenous retroviruses were observed. These results showed that the initial phase of HTLV-1 infection toward SGECs of SS was mediated not by viral synapses, but by biofilm-like components. |
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書誌情報 |
Virus Research 巻 269, p. 197643, 発行日 2019-06-21 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01681702 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.virusres.2019.197643 | |||||
権利 | ||||||
権利情報 | c 2019 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Virus Research, 269, art.no.197643; 2019 |