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Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice
http://hdl.handle.net/10069/16918
http://hdl.handle.net/10069/16918f108a75b-bbd2-4e3b-b3e0-6032c940c73b
名前 / ファイル | ライセンス | アクション |
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EJPB69_633.pdf (286.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-06-02 | |||||
タイトル | ||||||
タイトル | Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Targeting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Stomach | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mouse | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luciferase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nishi, Junya
× Nishi, Junya× Fumoto, Shintaro× Ishii, Hiroki× Kodama, Yukinobu× Nakashima, Mikiro× Sasaki, Hitoshi× Nakamura, Junzo× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Stomach-selective gene transfer is a promising approach as a therapeutic strategy for refractory gastric diseases. In this study, we improved the stomach selectivity of gene expression following microinstillation of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. pDNA encoding firefly luciferase was used as a reporter gene. It was confirmed that the gene expression level in the stomach 6 h after gastric serosal surface microinstillation of pDNA was significantly higher than after intragastric, intraperitoneal and intravenous administration. Regarding selectivity of gene expression, the gene expression level in the stomach after gastric serosal surface microinstillation of 1 μg/1 μL (dose/volume) pDNA was 5.7 times higher than that in the spleen. In our previous study (30 μg/30 μL), the expression level in the stomach was 2.7 times higher than that in the spleen; therefore, the selectivity was 2.1 times higher in this study. When we investigated gene expression at various pDNA solution concentrations, the ratio of the gene expression level in the stomach to that in the spleen was the highest as 1 μg/1 μL of pDNA, which was considered the optimal concentration. Information in this study is useful for further development of target organ-selective gene delivery systems. |
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書誌情報 |
European Journal of Pharmaceutics and Biopharmaceutics 巻 69, 号 2, p. 633-639, 発行日 2008-06 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09396411 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10836618 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ejpb.2007.12.018 | |||||
権利 | ||||||
権利情報 | Copyright c 2007 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | European Journal of Pharmaceutics and Biopharmaceutics, 69(2), pp.633-639; 2008 |